BIOS E-12 Principles and Techniques of Molecular Biology
Spring Term 2017 CRN 22965
Students gain in-depth knowledge of nucleic acid structure, molecular genetics, and the biochemistry of transcription and protein synthesis. Working from this foundation, students explore mechanisms of gene regulation in prokaryotes, eukaryotes, and viruses. The roles played by gene regulation and rearrangement in diseases are also examined. One large project comprises three linked laboratory exercises that introduce students to important recombinant DNA and protein expression techniques. Students learn about the construction of an expression plasmid and assays for normal promoter function.