BIOS E-176 Experimental Molecular and Cellular Biology
This course is designed to maximize student exposure to critical experimental techniques. The molecular biology portion of the lab includes a two hybrid screen for proteins that interact with p53. The candidate proteins are overexpressed, purified, and subsequently used in an electrophoretic mobility shift assay. In the cell biology portion, students perform experiments using two different model organisms. In Saccharomyces cerevisiae, budding yeast, students evaluate the conditions under which a transcriptional activator is transported in and out of the nucleus. In Dictyostelium discoidem, a slime mold, students evaluate the effect of a myosin mutant on cell motility and differentiation under starvation conditions. Prerequisites: BIOS E-12 and BIOS E-16/W, or the equivalent.
January session (23660)
Cheryl D. Vaughan, PhD, Lecturer on Molecular and Cellular Biology, Harvard University.